This test was developed to detect microorganism or anythings targets
from human, animal, food or environmental samples with simple, fast, high
specificity and precision. In the NucleoGene Molecular Detection Assay
(MDA) Kit method uses Circular Amplification Technology (CAT
METHOD), the amplification reaction at a constant temperature proceeds
under the isothermal condition. The reaction takes place at high
amplification efficiency with a plurality of 10 primers (all other different
from techniques) specific to the target gene region without the cycle, so
Real Time PCR (hydrolysis probes and hybridization methods) and
according to conventional PCR method is completed in less time. The
method has high tolerance to inhibitors, thus the human, animal, food and
environmental matrix effect is minimized. With its simple applicability, the
analysis is completed in 2 steps and in total a maximum of 45 minutes
(varies between kits). Amplification of these nucleic acids is carried out by
the CAT method using specific gene region-specific primers capable of
detecting all target. The presence of microorganism can be easily
determined by real-time monitoring of amplification curves in NucleoGene
Molecular Detection Assay Instrument. With the specially designed
NucleoGene Molecular Detection Instrument or the Real Time PCR (0,2
ml PCR tube or strip compatible), the results do not require
electrophoresis or any other method. All steps from amplification to
detection are carried out in a reaction tube. NucleoGene COVID-19 MDA Detection Kit is a real-time RT
amplificationbased detection system for the 2019 Wuhan coronavirus
(2019-nCoV). 2019-nCoV is considered a novel human coronavirus that is
genetically distinct from the common human coronaviruses (229E, NL63,
OC43, HKU1), which cause seasonal acute respiratory illness. It is also
genetically distinct from the two newer human coronaviruses, MERS-CoV
and SARS-CoV.